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Phalaenopsis Cornucervi Germination

This is a discussion on Phalaenopsis Cornucervi Germination within the Breeding & Hybridization forums, part of the Orchid Propagation category; Originally Posted by Ryan.Walsh Charlaux, Does your media contain anything other than agar? I ask ...

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  1. #11
    charlaux is offline Member
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    Quote Originally Posted by Ryan.Walsh View Post
    Charlaux,

    Does your media contain anything other than agar? I ask because orchid seeds won't just germinate on agar. The agar is just put into the mixture to solidify it. Orchid seeds require nutrients and most importantly carbon in order to germinate. In the wild these are provided by a symbiotic relationship between a fungi and the seed. In vitro we supply this by adding carbon in the form of sugar as well as varying amounts of nutrients (depends on the media). The main reason I ask this is because you stated you didn't sterilize the seeds but you don't have any contamination. Non-sterilized seeds in a normal orchid sowing media will almost immediately grow contaminants due to the high sugar and nutrient contents.

    Also Phalaenopsis seed is incredibly short lived. The most success is found from seed from a capsule that just split or from green pod sowing.
    Hi,
    The solution that I bought included,
    Ammonium Nitrate, 6-Benzylaminopurine, Boric Acid, Calcium Chloride Anhydrous, Cobalt Chloride Hexahydrate, Cupric Sulfate Pentahydrate, Disodium EDTA Dihydrate, Ferrous Sulfate Heptahydrate, Magnesium Sulfate Anhydrous, Manganese Sulfate, MES (Free Acid), Myo-Inositol, a-Naphthaleneacetic Acid, Nicotinic Acid (Free Acid), Peptone Type I, Potassium Iodide, Potassium Nitrate, Potassium Phosphate Monobasic, Pyridoxine Hydrochloride, Sodium Molybdate Dihydrate, Thiamine Hydrochloride, Sucrose & Zinc Sulfate Heptahydrate PLUS anti fungal agent and a custom blend of antibiotics

    I don't know maybe the seeds are infertile because It is my second flask and the first flask is contaminated.
    Again thank you

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    PLUS anti fungal agent and a custom blend of antibiotics
    Ah that would explain it. I'm not really sure about the effects of the antibiotics/antifungals on germination as the only one I've ever used is a product called PPM. Like I said though Phals are notoriously short lived. On the pods I've done, even storage in refrigeration for a month yields 0% versus very high being sown immediately after germination. Most seed is not like this though, Cattleya alliance for example stores quite well.

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    Best of luck to you in this endeavor!

    cheers,
    BD

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    Quote Originally Posted by charlaux View Post
    Hi,
    The solution that I bought included,
    Ammonium Nitrate, 6-Benzylaminopurine, Boric Acid, Calcium Chloride Anhydrous, Cobalt Chloride Hexahydrate, Cupric Sulfate Pentahydrate, Disodium EDTA Dihydrate, Ferrous Sulfate Heptahydrate, Magnesium Sulfate Anhydrous, Manganese Sulfate, MES (Free Acid), Myo-Inositol, a-Naphthaleneacetic Acid, Nicotinic Acid (Free Acid), Peptone Type I, Potassium Iodide, Potassium Nitrate, Potassium Phosphate Monobasic, Pyridoxine Hydrochloride, Sodium Molybdate Dihydrate, Thiamine Hydrochloride, Sucrose & Zinc Sulfate Heptahydrate PLUS anti fungal agent and a custom blend of antibiotics

    I don't know maybe the seeds are infertile because It is my second flask and the first flask is contaminated.
    Again thank you
    Wow that a lots of mineral including plants hormone almost complete, the other think you need to observed is the pH for the germination basically it's required between 5 to 6.5 relatively acidic usually we use KOH and HCL to adjust the the pH. based on my observation for cornu cervi, bellinas, amabilis usually after 2 weeks of sowing we can see greenish coloration of the seeds than by one month green color more prominent. Well using agar not all the orchids seeds they love that media that we are prepared some refuse at all but others germinated like no body business we have to do try an error in order to get proper/ correct media unless you buy ready made from recommended by friends. Never give up may be try reducing some of the minerals. or try this recipe:
    Peptone (50 to 100gm) + nearly ripe banana one is more than enough (blended get the juice) + fertilizer with N:P:K 21:21:21 ( one teaspoon) and plants hormone 6 Benzylaminopurine or a=Naphthalenacetic Acid ( dont touch with bear hand) try to make it 1 liter solution and sterilized.

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