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sterile water

This is a discussion on sterile water within the Flasking Equipment & Technique forums, part of the Orchid Propagation category; can i use pressure cooker to sterilise knives, forceps. and also prepare sterile water? what ...

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  1. #1
    Robert is offline Senior Member
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    Default sterile water

    can i use pressure cooker to sterilise knives, forceps. and also prepare sterile water? what is the length of time required?

  2. #2
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    Good question; from what I hear some use a torch, some soak their tools in Physan-20, there are a number of methods. Perhaps everyone will pipe in with their favorite methods of steralizing their tools.

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    I typically sterilize my media for about 25 min in the pressure cooker. As far as the equipments such as scalpels, I dip them in isopropyl alcohol, then flame them with a torch, just as Connie mentioned.

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    I use bleach, I soak instruments in bleach. It is also good to rinse your hands in bleach water between handling plants when you are opening wounds in them - anytime you might have a chance of transferring a fungus.

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    Quote Originally Posted by Germinatorman View Post
    I use bleach, I soak instruments in bleach. It is also good to rinse your hands in bleach water between handling plants when you are opening wounds in them - anytime you might have a chance of transferring a fungus.
    We do the torch thing with knives and tools and use bleach water as Don mentions.

    Cheers,
    BD

  6. #6
    Robert is offline Senior Member
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    Thanks for the feedbacks. I do dip the instruments in alcohol. My question was does sterilising instruments in pressure cooker, for an hour, kill the germ, or it's not necessary as using 70%(?) bleach was good enough.

    Another question of using alcohol. Is dipping without flaming effective to kill the foreign matters?

    I am using a glove hood.

  7. #7
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    Quote Originally Posted by Robert View Post
    Thanks for the feedbacks. I do dip the instruments in alcohol. My question was does sterilising instruments in pressure cooker, for an hour, kill the germ, or it's not necessary as using 70%(?) bleach was good enough.

    Another question of using alcohol. Is dipping without flaming effective to kill the foreign matters?

    I am using a glove hood.
    Robert, pressure heat sterilization of your instruments should not take more than 20 mins. Dipping without flaming is not effective, unless you soak the instruments for atleast half an hour or so.

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    Robert is offline Senior Member
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    Halloamey, thanks for the tips.

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