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experimenting with flasking media

This is a discussion on experimenting with flasking media within the Flasking Equipment & Technique forums, part of the Orchid Propagation category; Originally Posted by coeruleo what an amazing thread. i've wanted to try this myself, but ...

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  1. #61
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    Quote Originally Posted by coeruleo View Post
    what an amazing thread. i've wanted to try this myself, but it seems so daunting. i emailed a person selling flasking medium, and he was sort of rude about just how terribly difficult it is and asked if i had an autoclave (does anyone???) as you cannot do this without one supposedly. he wouldn't event try to suggest which of the 30 or so flasking mediums he offered i should try. he also asked where i was planning on getting the mycorrhizal fungus that was required... these things germinate in jungles in decidedly non-sterile nature, i just don't see how flasking has to be so damn technical.
    try it a few times, it is not that difficult actually. autoclave, no. i dun have one at home, i used double boiled method after mixing the media up and pour them into individual bottle and then double boiled them for 30 mins. i had friends who manage to do it with microwave.
    i do not know which media is the best but was told by fellow members of the forum that p668 works for most orchids so i decided to try with this. moreover i think it is one of the cheapest around.

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    Quote Originally Posted by Scorpio55 View Post
    I experimented with home-made mediums and believe me, you can do it yourself. I also obtained some commercial mediums. I have sown about 50 species now, and some germinate better on my home-made ones, some on the commercial ones, and some did not germinate at all so far. I keep a log, so if you are interested, just let me know, I'll give you the URL to my site. I have started this because I read on a thai site that people at home could do this ( just to be able to get a source of living), so it had to be quite simple. I have to be honest: I had contaminations in my jars, but never that much that I wanted to quit. I have had much fun observing what little changes in my mediums made in the growing plants. Life is too short to find that "perfect" medium. So if you would like to start: just do it but be aware of the fact that it works as an addiction: I have over 600 jars after 1,5 year.......
    OH MY GOD。。。600 over jars!!!
    also would like to have the url of yours. very interested to learn more about home made medium.

  3. #63
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    Default replatting 。。。。。。

    well, here it goes. my first replatting. but at the mid of doing it, my back is killing me and i was asking why am i doing this and also i will be thinking no more flasking for me!
    but looking back (the second and the third photo) on the final result.........hehe...i think i might do it again!!! haha

    but i was so tired in the mid of it that i think i broke sterility....keeping my finger cross here
    Attached Images Attached Images    

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    Look very neat, plenty of babies coming up.

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    Your seedlings will grow leaves initially, before roots. The roots will come in about 2 months or so after germination.
    Commercial bought media is normally PH balanced and should work fine in most cases. As said earlier, some are more effective on different genera.
    But there is commercial seed germination media too.

    If you are going to get contamination, you would normally see if within the first week.

    As for sugars, Potato grows roots, and banana grows leaves, while Coconut proliferates. You shouldn't have to worry about any of them with commercial media's.

  6. #66
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    Quote Originally Posted by plucker View Post
    Your seedlings will grow leaves initially, before roots. The roots will come in about 2 months or so after germination.
    Commercial bought media is normally PH balanced and should work fine in most cases. As said earlier, some are more effective on different genera.
    But there is commercial seed germination media too.

    If you are going to get contamination, you would normally see if within the first week.

    As for sugars, Potato grows roots, and banana grows leaves, while Coconut proliferates. You shouldn't have to worry about any of them with commercial media's.
    thank you so much for the info, well, as i said earlier that i suspected i broke aseptic technique and it did show during the very first week of replatting. about 75%of the flask are contaminated with bacteria. well, at least i got five flask left, it the number holds.
    thank you so much everyone for the info, i had learn so much from here.

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    more updates after the replating
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    Look good, Ian. They soon will produce leaves.

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    Just bear in mind that banana inhibits germination. You can also add some peptones for terrestrials and Paphipoedilum . Agar should be around 6-12 mg/litre. Best of luck !!

    ---------- Post Merged at 12:37 PM ----------

    Why not add some activated charcoal ? It binds a lot off minerals .

  10. #70
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    Quote Originally Posted by lcas2012 View Post
    Just bear in mind that banana inhibits germination. You can also add some peptones for terrestrials and Paphipoedilum . Agar should be around 6-12 mg/litre. Best of luck !!

    ---------- Post Merged at 12:37 PM ----------

    Why not add some activated charcoal ? It binds a lot off minerals .
    thanks for the additional info on the peptones......

    the media P668 i used had the activated charcoal inside, so far the results are consistent for my catasetums, very pleased with the results. i only add about 60g of banana per liter of the P668 media to make it into replatting medium.

    also experimented on two flask with low germination rate (only one to two protocorns per flask) to not replate them. just wanna see how well can they develop with only P668 alone. so far they produce longer shoots and less leaves.

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