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Flask Sterilization

This is a discussion on Flask Sterilization within the Flasking Equipment & Technique forums, part of the Orchid Propagation category; I do not own a pressure cooker or autoclave, or have access to one... I ...

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  1. #1
    girldesign is offline Junior Member
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    Question Flask Sterilization

    I do not own a pressure cooker or autoclave, or have access to one... I have been wondering if anyone has ever tried a placing the flasks in a hot water bath, like with canning jam, to sterilize them?

  2. #2
    Halloamey's Avatar
    Halloamey is offline Senior Member
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    You can sterilize empty glass ware and metal objects in a simple oven at 220 oC for 3 hours. But unfortunately for media steam sterilization in a pressure cooker or an autoclave is the only way. Media has to get to a temperature of 121 oC for atleast 15 to 20 minutes to be completely sterilized and without without pressure that temperature cannot be attained. One other way which I have personally used is the use of a microwave oven. First steam the microwave by boiling some water in it for about 5 to 10 minutes. Then I added media (150 ml) to my bottles and then microwaved them on high (900 w) for 3 minutes. It worked for my dendrobium and phal bud cultures, but the test was performed on a statistically insignificant sample size (8 bottles) so the results do not infer much. Hope it helps.

  3. #3
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    Brutal_Dreamer is online now Dreaming with my eyes open...
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    Quote Originally Posted by Halloamey View Post
    You can sterilize empty glass ware and metal objects in a simple oven at 220 oC for 3 hours. But unfortunately for media steam sterilization in a pressure cooker or an autoclave is the only way. Media has to get to a temperature of 121 oC for atleast 15 to 20 minutes to be completely sterilized and without without pressure that temperature cannot be attained. One other way which I have personally used is the use of a microwave oven. First steam the microwave by boiling some water in it for about 5 to 10 minutes. Then I added media (150 ml) to my bottles and then microwaved them on high (900 w) for 3 minutes. It worked for my dendrobium and phal bud cultures, but the test was performed on a statistically insignificant sample size (8 bottles) so the results do not infer much. Hope it helps.
    Thanks Amey!

    cheers,
    BD

  4. #4
    girldesign is offline Junior Member
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    Halloamey..thanks for such a wealth of information!! I guess I will search for a pressure cooker, and maybe try the microwave method!
    Posted via Mobile Device

  5. #5
    girldesign is offline Junior Member
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    Yay! My father in law got me a pressure cooker he found for cheap...so I'm now going to be sterilizing in style!
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