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how should I conduct the shoot formation from orchid callus experiment??

This is a discussion on how should I conduct the shoot formation from orchid callus experiment?? within the Flasking Equipment & Technique forums, part of the Orchid Propagation category; I grow the species of Epidendrum from seeds, but some seeds seems to become callus ...

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  1. #1
    susan zhang is offline Junior Member
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    Default how should I conduct the shoot formation from orchid callus experiment??

    I grow the species of Epidendrum from seeds, but some seeds seems to become callus and some callus would turn brown and dead if stay too long in the bottle. So I want to transbottle these callus and make them grow to plantlets.


    When I transbottle the callus should I devide these callus or just move one clump callus to a new bottle? Would one clump of callus form several plants or it depents on the amount of hormone that I add to the vitro?

    I'd better transbottle these callus before they turn brown. Could anyone give me some advice? I can't find much information about the shoot formation from callus on the internet, so any help would be great

    By the way this is my first time posting in this forum, I don't know how to post my picture here. I have uploaded my picture, but I can't drag it to the other box, can anyone tell me how to add a picture from my computer?

  2. #2
    Brutal_Dreamer's Avatar
    Brutal_Dreamer is offline Dreaming with my eyes open...
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    Hello. If you have uploaded a photo, then it will be in your 'manage attachments' window. Reply to your own thread by choosing the "go advanced" reply button at the bottom right of the page. Here, you will get the advanced editor. Click in the message, then click on the paper clip icon and then in the pop-down, you should see the name of the photo you uploaded (if indeed the image has been uploaded). Select the image and it will be inserted into your reply.

    cheers,
    BD

    Also check out the link in my signature below on posting photos.

  3. #3
    susan zhang is offline Junior Member
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    susan zhang is offline Junior Member
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    I tried your way but finally I use the basic loader to load my picture, thanks anyway.

    ---------- Auto-Merged DoublePost - Post added at 08:33 PM ---------- Previous post was at 08:30 PM ----------

    the picture is below this post.

    ---------- Auto-Merged DoublePost - Post added at 08:34 PM ---------- Previous post was at 08:33 PM ----------

    [QUOTE=susan zhang;265598]I tried your way but finally I use the basic loader to load my picture, thanks anyway.[COLOR="Silver"]

    [SIZE=1]---------- Auto-Merged DoublePost - Post added at 08:33 PM ---------- Previous post was at 08:30 PM ----------[

    ---------- Auto-Merged DoublePost - Post added at 08:36 PM ---------- Previous post was at 08:34 PM ----------

    sorry I'm trying to post my picture and make some mistakes here.

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    Robert is offline Senior Member
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    Quote Originally Posted by susan zhang View Post

    When I transbottle the callus should I devide these callus or just move one clump callus to a new bottle? Would one clump of callus form several plants or it depents on the amount of hormone that I add to the vitro?

    I'd better transbottle these callus before they turn brown. Could anyone give me some advice? I can't find much information about the shoot formation from callus on the internet, so any help would be great
    callus turning brown isn't unusual. As they grow and multiply it's time to transbottle/replate.

    you may cut/divide the number of callus accordingly and placed into appropriate flasks. you may also want to let them developed into plantlets or further multiplication for more callus.

    For shoots and roots formation Naa and Bap are adjusted accordingly ie more Naa and less Bap for roots formation and vice versa. you have to play around with the concentration as different seeds response differently to plant hormones.

  6. #6
    susan zhang is offline Junior Member
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    Thanks Rob! I'm going to devided them up today, I don't have BAP available so I use 6-BA instead. After I read some articles I add 3mg/ L NAA and 0.5mg/6-BA to the vitro.

    Last time when I transbottle my phaleanopsis which just started grow their first one or two leaves I added about 5mg/L NAA and 0.5mg/L 6-BA, the result is many of them grow longer roots before their leaves grow bigger, some leaves are also turning brown and lots of potocorns are turning brown seems to be burned by the high concentration of hormones. What's your thought of this problem?

    Here is the picture of my phaleanopsis.

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    After I transbottle the callus I will post the picture here.

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    Robert is offline Senior Member
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    Hi Susan, for roots i add 1mg/L Naa and Bap 0.5mg/L. For shoots 2mg/L Bap and 0.5mg/L Naa. This work well so far. But as i mentioned different plant work differently. Lots of trial and error. Ask around for other opinion.

    I read in an online journal the usage of " NAA supplemented medium, the callus was pale, yellowish green in colour more friable, hard and granular. But in IAA and IBA supplemented media, the callus was solid in nature and pale green in colour". In your present 3mg/L NAA if the result was still the same although you later reduce down further the concentration, i would suggest adding IAA instead. Just my though.
    Last edited by Robert; October 21st, 2011 at 04:39 AM. Reason: add extra sencences

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