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  • 1 Post By siaogu
  • 1 Post By siaogu

My attempt at hybridization - flasking medium

This is a discussion on My attempt at hybridization - flasking medium within the Flasking Equipment & Technique forums, part of the Orchid Propagation category; Recently, I had a lot of seed pods popping out from my plants. I borrowed ...

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  1. #1
    siaogu is offline Bulbolific bulbolicious
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    Default My attempt at hybridization - flasking medium

    Recently, I had a lot of seed pods popping out from my plants. I borrowed the lab for a day and tried to do my own flasking. This is how it goes.

    Ingredients
    MURASHIGE & SKOOG (MS) BASAL SALT MIXTURE
    Banana
    Sucrose
    Activated Carbon
    Agar - Molecular Grade


    MURASHIGE & SKOOG (MS) BASAL SALT MIXTURE


    MS mixture, activated carbon and sucrose


    Blending all the ingredients together


    The final mixture


    Regulating the pH of the mixture


    Splitting the mixture into flasks

    After this point, I added 0.5g of agar to each flask. I forgot to take pictures of this step. After mixing, I sealed the flasks with 2 layers of aluminium foil.


    The autoclaves


    Placing the flasks in the autoclave


    Setting the autoclave

    After this, the medium is cooled in room temperature, ready for the green pod germination which I did not document as i have to be totally sterilized while doing that. My hands are busy inside the Laminar Flow Work Station.

    These are the items needed for green pod germination

    Items
    Seed pods
    70% etOH
    100% etOH
    Scalpel and blade
    Long tweezer
    Alcohol burner
    Sterile petri dishes

    The 70% ethanol is used to wipe down the work stations as well as cleaning the seedpods before flaming them. The 100% ethanol is used to sterilize the work equipment like tweezers and blade. You just need to clean the pod thoroughly, slice them length wise and always keep every step sterile.


    Dendrobium flasks

    I placed 2 flasks of random dendrobium hybrid under infrared light as well as 5 flasks of Bulbophyllum makoyanum and 5 flasks of Bulbophyllum maxillare under fluorescent plant light. I have yet to go back to see the result, i had my fun, the result does not matter much

  2. #2
    catasetum-ian's Avatar
    catasetum-ian is offline apprentice
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    Default

    WOW...............cool. with all those equipment!!
    Vincent, when you said green seed pod, how green is green? meaning how soon can we harvest it for flasking?
    how do we know it is mature enough to be flasked?

  3. #3
    siaogu is offline Bulbolific bulbolicious
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    Default

    Quote Originally Posted by catasetum-ian View Post
    WOW...............cool. with all those equipment!!
    Vincent, when you said green seed pod, how green is green? meaning how soon can we harvest it for flasking?
    how do we know it is mature enough to be flasked?
    What my prof told me was that the yield will be better if the seed pod is close to its matured state. When the seed pods mature, the seeds will be more loosely packed than younger seed pods. Then again, he also said that you still will be able to get some plants out even if the seed pod is young.

  4. #4
    kiwiorchids's Avatar
    kiwiorchids is offline Plant Nut
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    omg, that isnt a kitchen.... 0_o

  5. #5
    catasetum-ian's Avatar
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    Quote Originally Posted by siaogu View Post
    What my prof told me was that the yield will be better if the seed pod is close to its matured state. When the seed pods mature, the seeds will be more loosely packed than younger seed pods. Then again, he also said that you still will be able to get some plants out even if the seed pod is young.
    thanks, that info is very useful to me. arigato!!!

  6. #6
    siaogu is offline Bulbolific bulbolicious
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    Quote Originally Posted by catasetum-ian View Post
    thanks, that info is very useful to me. arigato!!!

    No prob, we can talk offline on this topoc if you like

    Cheers
    Vincent

  7. #7
    catttan's Avatar
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    Hey Vincent - very cool equipment. Looks really hi-tech. Hope you produce some super crosses.

  8. #8
    siaogu is offline Bulbolific bulbolicious
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    Quote Originally Posted by catttan View Post
    Hey Vincent - very cool equipment. Looks really hi-tech. Hope you produce some super crosses.
    I have these seed pods in production:

    1. Phalaenopsis Seletar Tiger X Phalaenopsis fuscata
    2. Cattleya mesquitae X Brassavola nodosa
    3. Bulbophyllum maxillare X self
    4. Bulbophyllum makoyanum X self
    5. Bulbophyllum foetidum X self
    6. Bulbophyllum grandiflorum X Bulbophyllum longisepalum

    Hope I can get some successful flasks from these pods....

    Vincent

  9. #9
    Brutal_Dreamer's Avatar
    Brutal_Dreamer is online now Dreaming with my eyes open...
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    Vincent, I have promoted this to an article for the forum library. Thanks for the good work and information.

    cheers,
    BD

  10. #10
    catasetum-ian's Avatar
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    Quote Originally Posted by siaogu View Post
    No prob, we can talk offline on this topoc if you like

    Cheers
    Vincent
    tqvm for the info, will PM you when i needed more info. Merci beaucoup!!

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