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Encyclia tampensis germination

This is a discussion on Encyclia tampensis germination within the Flasking Equipment & Technique forums, part of the Orchid Propagation category; Hi everyone, Just thought I would share some pictures of the germination I've gotten with ...

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  1. #1
    Ryan.Walsh is offline Junior Member
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    Default Encyclia tampensis germination

    Hi everyone,

    Just thought I would share some pictures of the germination I've gotten with Encyclia tampensis. This seed was acquired from the seller in dried capsules and stored in the refrigerator after receipt. Seed was sterilized in 10% bleach solution+drop of dish soap for 10 minutes with frequent agitation in a 3cc syringe. After sterilization the seed was rinsed 3 times with sterile distilled water and dispensed into the flask (baby food jar with tissue culture caps) containing roughly 25ml 1/2 strength P668 media + 10% coconut water and 5 g/l sucrose added. Everything was done in a homemade sterile glove box sterilized with 10% bleach. Seeds were sown on February 21, 2013 and pictures are from today. Amazingly fast germination, some of the fastest I've seen with dried seed. Germination rate appears to be very high from the lack of visible ungerminated seeds present.

    Within the next week or so I'll be moving these over to spread replates with 3/4 strength P668, 5 g/L sucrose and banana added. The addition room to grow, higher nutrient strength and banana will speed up growth and prevent browning out that is often rampant in Cattleya alliance protocorms.







    I also have some Brassavola nodosa sown from greenpod at the same time that are starting to green up and quite a few more that look like they are in the early stages of germination.

    I'd be happy to answer any questions.

  2. #2
    King Kjeldz is offline Senior Member
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    good luck!! i have some seedpods on an encyclia right now...my first encyclia hybrids...ive never flasked dry seed..but from what i hear..many have stopped because of spores fitting into the hairs on the dry seed...hope your stringent cleansing works for u..i use green seedpods..here is some of my flaskings and also the darkest E.cordigera ive ever seen..its line bred and i wanna show it one day

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  3. #3
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    Nice going!!

    cheers,

    BD

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    You make it seem so easy ... and I know it's not so I'm impressed right out of my socks. I'm looking forward to seeing photo's of the mature plant someday soon. - martha

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    Wow good luck looking the out come.

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    I don't think I'll ever attempt this, so I'm doubly, triply impressed. Thank you for letting us see your expertise. I really enjoy watching and learning from everyone.

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    Wow - and I thought I knew a little about flasking - obviously, I don't! I'll be watching with great interest.

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    Thank you everyone for your kind words. It really isn't that hard of a process once you get the hang of it. I highly encourage any of you to start up if you are so inclined. I did replate some of the Encyclia tampensis as I had mentioned earlier but not nearly all of them. I have really gotten fantastic germination from these and admittedly underestimated the number of replate flasks I needed Upon opening up the mother flask a lot of what you saw in the first picture was stacked 5-10 deep in protocorms. I don't know yet how many of these I will ultimately want (for myself or to sell) so I haven't done much more with them. They are growing like weeds (albeit small weeds) and each protocorm has doubled in size, at least, in the last week.

    Aside from that I thought I would share a few other pictures with you all. Here is a Brassavola nodosa starting to germinate (the green dots) from green pod harvested at 12+ months post pollination. I have 4 more of these mother flasks and each flask is germinating slowly with new protocorms popping up every day. This one came from a selfing of a plant in the greenhouse at the University I'm finishing my Ph.D. at. From what I gather the mother plant is 20+ years old, I'll have to get a picture at the next blooming. I'm an adjunct Instructor there now but I used to teach Botany labs when I was a junior graduate student so the Horticulturalist and I are good friends. He lets me pollinate the species orchids in exchange for a few flasks along the way. As I mentioned this was green pod sown but the capsule maturation time is way beyond what is typically reported in the literature. The mother plant probably doesn't get enough light because it's shaded by a massive Staghorn fern so that could be part of the reason.


    Brassavola nodosa starting to germinate

    Ans here's a picture of my homemade glovebox I do all of my work in. I have plenty of access to laminar flow hoods in the department but being able to do the work in the comfort of my own home when I want to is invaluable. In total the whole thing probably cost me $20. The case is just a rubbermaid storage container with pvc fittings for the arm holes and cleaning gloves (around $2.00). Everything is sealed and sprayed with 20% bleach prior to work. The gloves have to be replaced quite frequently due to the bleach degrading them. A very cheap, easy to use alternative to the lab equipment I am used to using.


    My homemade glovebox

    King: yeah dry seed can be a pain sometimes but most of my experience with dry seed comes from necessity. I do my own crosses when I can but getting seed from outside sources of species I wouldn't normally have is also nice. The 10% bleach for 10 minutes usually takes care of the contamination issue but I usually use Plant Preservative Mixture (PPM) at 1 ml/L in the media as a fail safe in the dry seed sowings. Too bad you are out of the states because I would love a flask of that cordigera. Best of luck in your endeavors.

  9. #9
    Robert is offline Senior Member
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    very neat, successful flasking and setup you have there.

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    orchidnewb_dad is offline Junior Member
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    Hi everybody
    Just wondering if you might be able to give me a little help. I have a dendrobium flask with about 100 protocorms in it. I know very little about orchids other than what I have read on this forum. I started with 3 flasks all different species bought online. one got contaminated right off the start and one only just showed one protocorm after about 3 months(the dendrobium started germinating after a few weeks) I have purchased the replate medium but don't know when I should replate or how many little babies to put in each new flask. Also I have had them in front of a window since I started in late january(no direct sunlight) when can I start giving them sun or is fluorescent lighting better. Thanks in advance for any help you can offer.

    Cheers
    Posted via Mobile Device

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