Shop Orchid Care OrchidTalk Orchid Forum Weather Station Links Nursery

Welcome to OrchidTalk Orchid Forums


The Friendliest Orchid Community on the Internet!


  •  » Learn to Repot your Orchids
  •  » Learn Orchid Care Tips and Secrets
  •  » Find the perfect Orchid for your Growing Environment
  •  » Chat with Orchid Growing Professionals

OrchidTalk - "Bringing People Together to Grow Orchids Better!"


Let us help you grow your Orchids better; Join our community today.


YES! I want to register an account for free right now!


Register or Login now to remove this advertisement.

Page 1 of 4 123 ... LastLast
Results 1 to 10 of 31

Flasking Setup

This is a discussion on Flasking Setup within the Flasking Equipment & Technique forums, part of the Orchid Propagation category; Hi all, Just thought I'd share my flasking setup & a few pics of flasks. ...

Click here to increase the font size Click here to reduce the font size
  1. #1
    montanum is offline Junior Member
    Join Date
    Jun 2005
    Posts
    29

    Default Flasking Setup

    Hi all,
    Just thought I'd share my flasking setup & a few pics of flasks.

    I grow solely hardy terrestrials in-vitro. This, of course, means that all my flasks need to be in the dark for a year and a bit. So, I have my setup in the closet... I'm sure the police think I'm doing something else, but I swear, it's just orchids (no lights at all in there).

    Here's the closet, messy as it is otherwise. The Cabinet on the right is for mother flasks. I also have a bunch of flasks in the fridge that are due to come out today, finally! The boxes on top and the crate to the left are for replates. I also have a large number of replates in an empty dresser in the guest room. Everything is covered with light blocking blankets to cut any light that gets through the cracks in the closet doors.



    Below are several replate pictures, just for fun.
    Here's a Cyp acaule from about a month ago. It's grown very well.


    This is an older Cyp acaule from about 5 months ago. It is mature at the wrong time! since I replated at the wrong time... Notice the difference in bud size to the previous.


    A replate of Cyp californicum that I did 2 weeks ago. It's starting to grow again after being moved.

  2. #2
    montanum is offline Junior Member
    Join Date
    Jun 2005
    Posts
    29

    Default

    Here's Cyp passerinum which I did about 4 weeks ago, but they were smaller protocorms. It's still a mystery why some elongate their buds and some don't (preferable).


    Cyp Sebastian (parviflorum X montanum) which I did about 4 weeks ago.


    A Cyp flavum that has grown very well in 3 months.


    Here is Cyp montanum from 2 weeks ago.

  3. #3
    montanum is offline Junior Member
    Join Date
    Jun 2005
    Posts
    29

    Default

    And a Cyp montanum from 3 months ago. They grow slowly and form small buds for me, so far. We'll see what happens.


    And last, the oldest plants of the photos, a Platanthera hyperborea flask that has been replated a few times, and has been in the fridge for 3 months for winter, has been exposed to light so they grew out their buds and is theoretically ready to be outside... except that they are only now growing roots! I'll have to wait a while before I can plant them because of this.


    I'd love to see some of your flasking setups.

    Best,
    Ross

  4. #4
    Tindomul1of9's Avatar
    Tindomul1of9 is offline Senior Member
    Join Date
    Dec 2005
    Location
    NYC
    Posts
    896

    Default

    That is soo cool! What is the media? Agar? Also, why dont they get put in the light?

  5. #5
    montanum is offline Junior Member
    Join Date
    Jun 2005
    Posts
    29

    Default

    The media are Phytotech premade blends. I don't have a lab in which to mix my own (yet...) but they have proven to be suitable. I have made small additions to each. The basic replating medium for my Cyps is T842. The black medium is Malmgren's which I use for everything non-Cyp. You can see potato chunks in the flasks, which was a hint from Bill Steele (as well as were the media themselves, not to mention bleaching times).

    As for the light issue, when hardy orchids germinate in nature, they are under a layer of leaf litter, preventing light from reaching them. Aside from this natural motivation, if exposed to light they will elongate their buds and waste that energy that they put into them because they don't get to keep these leaves, unlike their tropical cousins. The best thing to do is to have the first bud to form be the only bud to have to form. The tiny amount of photosynthetic energy they would gain from their primordial leaf is insignificant compared with the abundant sugar/nutrient energy in the medium.

    Best,
    Ross

  6. #6
    Hoa Tony Nguyen's Avatar
    Hoa Tony Nguyen is offline Senior Member
    Join Date
    Nov 2005
    Location
    Pasadena and Costa Mesa
    Posts
    2,408

    Default

    Your flask pictures are amazing, especially how to raise the Cyp. You seem to have good ideas about how to keep things sterile for seed germination, free of those bothersome molds! Congrats on those sucessful experiments!
    Cheers. Hoa.

  7. #7
    bench72's Avatar
    bench72 is offline Moderator
    Real Name
    Tim
    My Grow Area
    Porch/Patio.
    Favorite Orchid(s)
    Paphiopedilums
    Join Date
    Jan 2005
    Location
    Sydney, Australia
    Posts
    5,480
    Member's Country Flag

    Default

    oh, they look so amazing.... I am quivering from the thoughts of those Cyp acaule, growing and maturing and flowering into those lovely blooms... (ok, maybe I'm shivering because it is cold here today )

    but what's really getting me going is the pic of plants replated at the wrong time... this is very important for the likes of me who will one day try and source these plants from the north and bring them south where the seasons are reversed. so, note to self, contact source of cyp acaule flask 5 months prior to pick up, and ask them to replate even though it is the wrong time!

  8. #8
    montanum is offline Junior Member
    Join Date
    Jun 2005
    Posts
    29

    Default

    Bench,
    Cyp acaule is ridiculous. I have never had good luck predicting its behavior. In different flasks, I've had some sporadic germination 3 months after sowing, I've had 90% germination 9 months after sowing, I've had ok germination anwhere from 2-5 months after sowing and I've had no germination ever in some flasks.

    This year, I was sure to be consistent so I could gain some cogent information pertaining to the species (I'm doing acaule alba this year, mostly). I bleached from 1hr to 2hr and various times between, and I refridgerated 5 months irregardless. It has now been 1 month since I have removed them from the fridge and they are starting to germinate. There should be enough and they should be of replatable size in about 3 months, I'm guessing. Hopefully this strict coersive regimen will induce more amicable and timely results.

    Easier species to grow for you might be formosanum and kentuckiense, due to their heat tolerance.

    Best,
    Ross

  9. #9
    Piper's Avatar
    Piper is offline Hangs
    Join Date
    Dec 2004
    Location
    Massachusetts
    Posts
    7,068

    Default

    Hi Ross,

    Great thread and incredible pictures! Is the refridgeration necessary because they're cyps and need to vernalize? Or would that come later?

    Julie

  10. #10
    montanum is offline Junior Member
    Join Date
    Jun 2005
    Posts
    29

    Default

    Hi Julie,
    I've described two types of refridgeration: pregermination and post germination. The post germination is their first years vernalization. It happens after they have grown to a suitable size (and have been removed from flask usually). I didn't remove the Platanthera before refridgeration because I knew the lack of roots would be a problem.

    The refridgeration directly after sowing can sometimes cause better germination than otherwise. I have begun to use it as a precaution with C. acaule since it's such a pain to germinate sometimes. Most others don't require it, but I had a problem with my C pubescens flasks not germinating in a timely way this year, so they all went in too. I took them out last friday from 3 months cold, and noticed some preliminary germination :-)

    Best,
    Ross

Page 1 of 4 123 ... LastLast

Similar Threads

  1. My orchid greenhouse and setup
    By virgilspells in forum OrchidTalk Members Grow Area - Photos
    Replies: 4
    Last Post: June 20th, 2009, 05:08 PM
  2. My Greenhouse setup
    By Hillbilly Bob in forum Semi Hydro / Lights / Greenhouses / Accessories
    Replies: 11
    Last Post: February 21st, 2009, 07:17 PM
  3. Guess What I bought at the Saginaw Valley Orchid Show (setup)
    By KevinGofMI in forum Paphiopedilum, Phragmipedium, Cypripedium IN BLOOM
    Replies: 12
    Last Post: November 27th, 2006, 02:00 AM
  4. New acquisitions and Setup (PHOTOS!)
    By wogga in forum General Orchid Culture
    Replies: 8
    Last Post: July 31st, 2006, 08:02 PM
  5. semi-hydro setup
    By Jmoney in forum Photography Archive 1
    Replies: 7
    Last Post: June 9th, 2004, 10:39 PM

Tags for this Thread

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
OrchidTalk --An Orchid Growers Discussion Forum brought to you by River Valley Orchidworks. A World Community where orchid beginners and experts talk about orchids and share tips on their care, cultivation, and propagation.