Tightly sealed flask

[phytogems]

After one (1 ) week, I observed that my homemade sowing medium is free from contamination, thus I plan to sow my orchid seeds. My question is, should I seal the flask tightly? What happened to the cultured inside the flask if there is no air/oxygen? Anybody can advise me what to do.

[jeffrey6115]

For initial protocorm developement no oxygen exchange is necessary. I only add a "breather" when I replate. I have some mother flasks that have original plants that are almost 2 years old. They still look fine. I have opened them only to remove plants for replating then closed again with original lid.

[Brutal_Dreamer]

For initial protocorm developement no oxygen exchange is necessary. I only add a "breather" when I replate. I have some mother flasks that have original plants that are almost 2 years old. They still look fine. I have opened them only to remove plants for replating then closed again with original lid.

Thanks for the information. I wondered about this before and thought maybe they made their own oxygen since most are sealed up to keep our pollutants.

Cheers,
BD

[GrumpyBear]

i've been under the assumption it's carbon they need and i thought that's what's up with the charcoal in the medium... what do i know tho, my only attempt at flasking successfully grew disgusting fuzz in large quantities.

...anyone need some fuzz?