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Thread: experimenting with flasking media

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  1. #1
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    ian
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    Default experimenting with flasking media

    just read a few post in OTF about home made culture medium. so decided to try out an experiment, modified the version given in one of the thread.

    ingredients
    1. 500ml coconut water + 500ml water
    2. one banana
    3. three medium size potato
    4. 1 inch by 1 inch coconut sugar
    5. 1/4 teaspoon 20:20:20 fertilizer
    6. 1/2 tablespoon Azomite (source of trace minerals)
    7. 1/4 pack of 260 gm agar

    i put ingredient 1 to 4 in a high speed blender (2 Hp) for about i minute (hoping to get more nutrients in the flask by doing so), then cooked the solution for about 20 mins, added the fert and Azomite during the last minute and then the agar finally and stir till it dissolved. i did not sieve the solution as the high speed blender made everything into nice thick slurry solution.


    had a doritis pulcherrima seed pod that is ready to harvest outside. will do the sowing tomorrow.
    well, i am a first timer in this, just like to experiment with things. will keep posting about the progress. hopefully can see some germination.
    will keep posting about the progress from time to time.
    looking forward for any comments.
    Attached Images Attached Images   

  2. #2
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    goodluck and congrats in advance.

  3. #3
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    Bruce Brown
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    Awesome. Good luck. I look forward to learning of your success!

    Cheers,
    BD

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    may i ask how and where you will sow the seeds inside the bottles? also how do you avoid contamination?

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    Default

    Hi,
    I´m interested.
    Have you seed growing in those mother flasks?
    Show us somw pictures.
    thanka.
    bye
    Michael from Venezuela

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    jean françois AGUILLON
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    first I tried this medium formulation ,just for looking, it is stuning indeed , a little bit slow in the beginning , I replated for the second time on it some phal's hybrid , strong roots as you can see, thanks for your post


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    Default Excellent

    Hi,
    Your flasks look amazing. One question: what is coconut sugar and azomite? can we replace them?
    Bye
    Mike

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    Quote Originally Posted by mikewalter View Post
    Hi,
    Your flasks look amazing. One question: what is coconut sugar and azomite? can we replace them?
    Bye
    Mike
    coconut sugar = natural sugar produced from sap obtained from cut flower buds of coconut
    ---- vendor link removed - see FAQs on posting ----

    and as for the update, unfortunately non of the doritis pulcherrima germinated, i asked a local orhcid nursery owner, he said the ingredient looks fine but he mentioned that i did not check the pH. he said the pH is very important. adjust it to about 5.5 with pH meter
    i am still keeping the flask (it has been about 4 months plus), although no growth but no contamination with fungal growth so i guess i will still keep for 2 more months.

  9. #9
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    Cathy
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    Looking forward to your continued posts on your experiment.

  10. #10
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    Zainal Abidin Bin Othman
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    Quote Originally Posted by catasetum-ian View Post
    coconut sugar = natural sugar produced from sap obtained from cut flower buds of coconut
    ---- vendor link removed - see FAQs on posting ----

    and as for the update, unfortunately non of the doritis pulcherrima germinated, i asked a local orhcid nursery owner, he said the ingredient looks fine but he mentioned that i did not check the pH. he said the pH is very important. adjust it to about 5.5 with pH meter
    i am still keeping the flask (it has been about 4 months plus), although no growth but no contamination with fungal growth so i guess i will still keep for 2 more months.
    Hi Ian, you can use KOH ( Potassium hydroxide) and HCL ( Hydrochloric acid) to adjust the pH do not use Naoh ( Sodium hydroxide) , I wish I can see some progress, good luck.

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