Orchid Care OrchidTalk Orchid Forum Links Nursery

Welcome to OrchidTalk Orchid Forums


The Friendliest Orchid Community on the Internet!


  •  » Learn to Repot your Orchids
  •  » Learn Orchid Care Tips and Secrets
  •  » Find the perfect Orchid for your Growing Environment
  •  » Chat with Orchid Growing Professionals

OrchidTalk - "Bringing People Together to Grow Orchids Better!"


Let us help you grow your Orchids better; Join our community today.


YES! I want to register an account for free right now!


Register or Login now to remove this advertisement.

Results 1 to 10 of 10

Thread: My attempt at hybridization - flasking medium

Click here to increase the font size Click here to reduce the font size
  1. #1
    Real Name
    Vincent
    My Grow Area
    Porch/Patio.
    Favorite Orchid(s)
    Bulbophyllums, Bulbophyllums
    Join Date
    Sep 2010
    Gender
    Male
    Location
    Singapore
    Posts
    1,227
    Member's Country Flag

    Default My attempt at hybridization - flasking medium

    Recently, I had a lot of seed pods popping out from my plants. I borrowed the lab for a day and tried to do my own flasking. This is how it goes.

    Ingredients
    MURASHIGE & SKOOG (MS) BASAL SALT MIXTURE
    Banana
    Sucrose
    Activated Carbon
    Agar - Molecular Grade


    MURASHIGE & SKOOG (MS) BASAL SALT MIXTURE


    MS mixture, activated carbon and sucrose


    Blending all the ingredients together


    The final mixture


    Regulating the pH of the mixture


    Splitting the mixture into flasks

    After this point, I added 0.5g of agar to each flask. I forgot to take pictures of this step. After mixing, I sealed the flasks with 2 layers of aluminium foil.


    The autoclaves


    Placing the flasks in the autoclave


    Setting the autoclave

    After this, the medium is cooled in room temperature, ready for the green pod germination which I did not document as i have to be totally sterilized while doing that. My hands are busy inside the Laminar Flow Work Station.

    These are the items needed for green pod germination

    Items
    Seed pods
    70% etOH
    100% etOH
    Scalpel and blade
    Long tweezer
    Alcohol burner
    Sterile petri dishes

    The 70% ethanol is used to wipe down the work stations as well as cleaning the seedpods before flaming them. The 100% ethanol is used to sterilize the work equipment like tweezers and blade. You just need to clean the pod thoroughly, slice them length wise and always keep every step sterile.


    Dendrobium flasks

    I placed 2 flasks of random dendrobium hybrid under infrared light as well as 5 flasks of Bulbophyllum makoyanum and 5 flasks of Bulbophyllum maxillare under fluorescent plant light. I have yet to go back to see the result, i had my fun, the result does not matter much

  2. #2
    Real Name
    ian
    My Grow Area
    Outside 24/7
    Favorite Orchid(s)
    catasetums
    Join Date
    Oct 2010
    Gender
    Male
    Location
    malaysia, selangor
    Posts
    1,218
    Member's Country Flag

    Default

    WOW...............cool. with all those equipment!!
    Vincent, when you said green seed pod, how green is green? meaning how soon can we harvest it for flasking?
    how do we know it is mature enough to be flasked?

  3. #3
    Real Name
    Vincent
    My Grow Area
    Porch/Patio.
    Favorite Orchid(s)
    Bulbophyllums, Bulbophyllums
    Join Date
    Sep 2010
    Gender
    Male
    Location
    Singapore
    Posts
    1,227
    Member's Country Flag

    Default

    Quote Originally Posted by catasetum-ian View Post
    WOW...............cool. with all those equipment!!
    Vincent, when you said green seed pod, how green is green? meaning how soon can we harvest it for flasking?
    how do we know it is mature enough to be flasked?
    What my prof told me was that the yield will be better if the seed pod is close to its matured state. When the seed pods mature, the seeds will be more loosely packed than younger seed pods. Then again, he also said that you still will be able to get some plants out even if the seed pod is young.

  4. #4
    Real Name
    Jordan Healey
    My Grow Area
    Greenhouse
    Favorite Orchid(s)
    The Weird and the Wonderful!
    Join Date
    Jul 2010
    Gender
    Male
    Location
    Hamilton, New Zealand
    Posts
    3,382
    Member's Country Flag

    Default

    omg, that isnt a kitchen.... 0_o

  5. #5
    Real Name
    ian
    My Grow Area
    Outside 24/7
    Favorite Orchid(s)
    catasetums
    Join Date
    Oct 2010
    Gender
    Male
    Location
    malaysia, selangor
    Posts
    1,218
    Member's Country Flag

    Default

    Quote Originally Posted by siaogu View Post
    What my prof told me was that the yield will be better if the seed pod is close to its matured state. When the seed pods mature, the seeds will be more loosely packed than younger seed pods. Then again, he also said that you still will be able to get some plants out even if the seed pod is young.
    thanks, that info is very useful to me. arigato!!!

  6. #6
    Real Name
    Vincent
    My Grow Area
    Porch/Patio.
    Favorite Orchid(s)
    Bulbophyllums, Bulbophyllums
    Join Date
    Sep 2010
    Gender
    Male
    Location
    Singapore
    Posts
    1,227
    Member's Country Flag

    Default

    Quote Originally Posted by catasetum-ian View Post
    thanks, that info is very useful to me. arigato!!!

    No prob, we can talk offline on this topoc if you like

    Cheers
    Vincent

  7. #7
    Real Name
    Yew-Sung
    My Grow Area
    Outside 24/7
    Favorite Orchid(s)
    cattleyas, vandaceous,paphios
    Join Date
    Sep 2009
    Gender
    Male
    Location
    Kedah, Peninsular Malaysia
    Posts
    16,047
    Member's Country Flag

    Default

    Hey Vincent - very cool equipment. Looks really hi-tech. Hope you produce some super crosses.

  8. #8
    Real Name
    Vincent
    My Grow Area
    Porch/Patio.
    Favorite Orchid(s)
    Bulbophyllums, Bulbophyllums
    Join Date
    Sep 2010
    Gender
    Male
    Location
    Singapore
    Posts
    1,227
    Member's Country Flag

    Default

    Quote Originally Posted by catttan View Post
    Hey Vincent - very cool equipment. Looks really hi-tech. Hope you produce some super crosses.
    I have these seed pods in production:

    1. Phalaenopsis Seletar Tiger X Phalaenopsis fuscata
    2. Cattleya mesquitae X Brassavola nodosa
    3. Bulbophyllum maxillare X self
    4. Bulbophyllum makoyanum X self
    5. Bulbophyllum foetidum X self
    6. Bulbophyllum grandiflorum X Bulbophyllum longisepalum

    Hope I can get some successful flasks from these pods....

    Vincent

  9. #9
    Real Name
    Bruce Brown
    My Grow Area
    Greenhouse
    Favorite Orchid(s)
    Cattleyas & Slippers
    Join Date
    Mar 2003
    Gender
    Male
    Location
    Arkansas
    Posts
    35,136
    Member's Country Flag

    Default

    Vincent, I have promoted this to an article for the forum library. Thanks for the good work and information.

    cheers,
    BD

  10. #10
    Real Name
    ian
    My Grow Area
    Outside 24/7
    Favorite Orchid(s)
    catasetums
    Join Date
    Oct 2010
    Gender
    Male
    Location
    malaysia, selangor
    Posts
    1,218
    Member's Country Flag

    Default

    Quote Originally Posted by siaogu View Post
    No prob, we can talk offline on this topoc if you like

    Cheers
    Vincent
    tqvm for the info, will PM you when i needed more info. Merci beaucoup!!

Similar Threads

  1. My Orchid Hybridization Project
    By siaogu in forum Breeding & Hybridization
    Replies: 13
    Last Post: November 8th, 2011, 12:29 AM
  2. Flasking Medium Suggestions
    By girldesign in forum Flasking Equipment & Technique
    Replies: 4
    Last Post: July 23rd, 2011, 11:42 AM
  3. Musings on Hybridization...
    By clintdawley in forum Breeding & Hybridization
    Replies: 1
    Last Post: May 31st, 2007, 08:40 PM
  4. First Attempt at S/H
    By Becky15349 in forum Semi Hydro / Lights / Greenhouses / Accessories
    Replies: 7
    Last Post: May 13th, 2007, 11:12 AM
  5. First attempt at flasking
    By LJA in forum Flasking Equipment & Technique
    Replies: 23
    Last Post: November 5th, 2006, 01:44 AM

Tags for this Thread

Posting Permissions

  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •  
OrchidTalk --An Orchid Growers Discussion Forum brought to you by River Valley Orchidworks. A World Community where orchid beginners and experts talk about orchids and share tips on their care, cultivation, and propagation.