do you all think this protocorns will survive?

[catasetum-ian]

just want some opinion about these protocorns. do you think they will make it??

i had to removed them from the mother flask because there is fungal contamination after i remove some of the protocorn for replating. they are only about 1-2mm big
i was just thinking....well, they had chlorophyll now, so should be able to make some food for themselves to survived...haha.
i had two protocorn removed from the replating flask last week and till today they still look green, that is why i took this drastic step.

finger crossed

[panam]

Oh, I hope so, Ian!

[zainal abidin]

As long as no infection from fungal/bacteria/virus and you should be able to maintain the humidity, no dryness of the moss theoretically the protocoms will survive anyway all the best good luck.

[catasetum-ian]

As long as no infection from fungal/bacteria/virus and you should be able to maintain the humidity, no dryness of the moss theoretically the protocoms will survive anyway all the best good luck.

many thanks for the encouragement.......mucha gracias!!!

[pavel]

Hope they survive.

I wonder if treating the protocorms with something like R-D-20 would be beneficial?

R-D-20
n-Alkyl (60%C14, 30%C16, 5%C12, 5%C18) dimethyl benzyl ammonium chloride ............ 10.0%
n-Alkyl (68%C12, 32%C14) dimethyl ethylbenzyl ammonium chloride .............................. 10.0%
INERT INGREDIENTS: .................................................. .................................................. . 80.0%
TOTAL: .................................................. .............................................. 100.0%

If you have anything similar, you might consider "experimenting" on a few of the protocorms. In any event, you certainly have nothing to lose by trying to keep them going.

[catasetum-ian]

Hope they survive.

I wonder if treating the protocorms with something like R-D-20 would be beneficial?

R-D-20
n-Alkyl (60%C14, 30%C16, 5%C12, 5%C18) dimethyl benzyl ammonium chloride ............ 10.0%
n-Alkyl (68%C12, 32%C14) dimethyl ethylbenzyl ammonium chloride .............................. 10.0%
INERT INGREDIENTS: .................................................. .................................................. . 80.0%
TOTAL: .................................................. .............................................. 100.0%

If you have anything similar, you might consider "experimenting" on a few of the protocorms. In any event, you certainly have nothing to lose by trying to keep them going.

thanks for the info, i had no idea where to get the rd 20 but i had rinse them with mancozeb. in addition, the mold were whitish and only infected part of the mother flask, i salvage those that grossly looks normal. also there are some forum members saying that those white hyphae might be a form of penicillin fungus that does no harm to the protocorns once removed.
so i am keeping my finger cross. haha ....

many thanks for all the response

[King Kjeldz]

Well goood f*cking luck...why not try stranger things have happened in this life.....a fungicide is definitely in order..high humidity I see u got that covered...cross ur fingers hope for the best and they would need some light
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[PaphMadMan]

DO NOT USE R-D-20 or any similar quaternary ammonium disinfectant on protocorms. Intact external tissues of mature plants are quite resisitant to this type of product, but protocorms are not much more than naked cells. No thick protective layers. Most actual fungicides should be reasonably safe though. Some can be used in plant tissue culture.

Protocorms can survive in situations like this, they certainly do in nature, but at this stage they would be reliant on symbiotic fungi for nutrients. Without a sterile environment it will be difficult to supply those nutrients without bacteria and fungi taking over. Good luck.

[catttan]

Good luck to you Ian.

[Orquiadicto]

Good luck and keep us posted.