Flasking -101

[Brutal_Dreamer]

This all looks very complex! Let us know how it works.

Cheers,
BD

[mycologist]

Think of it like instant cupcakes - just add water and warm to mix, then put it into the cups and bake. It took 2 hours total, and 90 min. of that was cooking time. Hagem's media would be on the souflee end - a bunch of stock solutions to prep and more than a dozen ingredients.

I was actually fairly dissapointed that I would not have to grow a fungus with the seeds.

[Brutal_Dreamer]

Think of it like instant cupcakes - just add water and warm to mix, then put it into the cups and bake. It took 2 hours total, and 90 min. of that was cooking time. Hagem's media would be on the souflee end - a bunch of stock solutions to prep and more than a dozen ingredients.

I was actually fairly dissapointed that I would not have to grow a fungus with the seeds.

Hahah... you are funny. Well, to an arts oriented guy, that all still looks pretty complex. I had a few flash-backs to bio lab when looking at the photos. I am happy it was easy for you and hope you have excellent results. I bet you will.

Cheers,
BD

[mycologist]

Hahah... you are funny. Well, to an arts oriented guy, that all still looks pretty complex. I had a few flash-backs to bio lab when looking at the photos. I am happy it was easy for you and hope you have excellent results. I bet you will.

Cheers,
BD

Thanks BD, glad you were quick to pick up on my dry humor.

The methods I am presenting are tried and true, although some adjustments may be necessary. So if it doesn't work I will have to take the blame!

The major items I am relying on are an autoclave (a big pressure cooker but you just press a button and come back later) and laminar flow hood (or biosaftey cabinet like mine). If you have any connection (or can make friends easily) with a biology department I would bet you could find someone with access who is interested (botanist, mycologist, or microbiologist). Otherwise, a flow hood just requires a hepa filter, a fan, and a working space. The filter goes in the back and the sterile air blows over the working area constantly. You can also use a "glove box".

You may want a drink before I post the next part.