Quote Originally Posted by jono
I've been looking all over the net and in books, but I'm still not clear on where to take the meristem explant from on the actual plant. I understand it's on the stem tip near the primordial leaves, but can someone point to a picture that actually shows this location or explain where this location can be (it seems it can be in a couple places on the plant).
Best I understand, it depends on the genus. Some you can do a root-tip meristem, where you simply remove the green tip from a root (and chop it up, and so forth and so on). With others, you need the actual plant growing tip, which is rather difficult to get to on monopodial growth habits, and requires at least wounding the plant, if not tearing it to bits, but is relatively easy and non-damaging with sympodial habits.

What genus are you working with, and what has your research turned up so far? I might not be able to help much, but perhaps somebody else will be able to.